A key research question is why the lipid composition of the cell membrane changes with age. The aging process seems to alter the composition of lipids (fats), the building blocks of cell membranes. As a result, the structural properties of the cell membrane change as well. The lipid composition of membranes plays an important role in regulating the activity of cell surface membrane receptors, which transmit external signals to the cell interior and thereby control cell fate. In their search for definitive answers, scientists at CECAD combine mass spectrometry of membrane lipids with genetically defined deficiencies for specific enzymes involved in lipid metabolism to gain new insight into the characteristics of age-dependent changes of the lipid composition of cellular membranes and their impact on age-related diseases secondary to loss of cell function. By analysing the cellular lipidome, CECAD scientists have succeeded in improving our understanding of the changes of lipid composition occurring in aging membranes in mice and humans. The structural and functional characterization of aging membranes is an important component of CECAD’s ultimate goal: to identify the mechanisms that lead to aging.
Research Area D investigates the following research topics:
CECAD expects these research activities will contribute considerably to the elucidation of triggers and progression factors driving aging-associated inflammation, neurodegenerative diseases, ceramide synthase-associated metabolic disorders, lipid-associated epidermal barrier function, mitochondrial-linked neurodegenerative diseases, and kidney failure.
Prof. Dr. Martin Krönke
Film (01:55, English)
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Figure 1: Lipid bilayer of cellular membranes
Figure 2: ER association of GFP-tagged Ceramide synthase 1 in COS cells (super resolution microscopy, stimulated emission depletion (STED))
Figure 3: Building blocks of the lipid bilayer
Figure 4 AND 5: Enzymatic activity and tissue specific expression of ceramide synthases 1–6