CECAD

Lipidomics/Metabolomics Facility

For more than 10 years, the Facility has offered a comprehensive service for the analysis of lipids in biological samples using state-of-the-art chromatographic and mass spectrometric (MS) techniques. Since the beginning of 2019, we have expanded our expertise to the analysis of polar and ionic metabolites. Our service covers the entire workflow from sample preparation and MS measurement of the relevant metabolites to processing and analysis of the raw data obtained. Since 2016, the CECAD Lipidomics/Metabolomics Facility has been registered in the DFG portal "RIsources", a portal for research infrastructures.

 

Dr. Susanne Brodesser, Dipl. Chem.
Head of the Lipidomics/Metabolomics Facility 
 +49 221 478 840 15
susanne.brodesser[at]uk-koeln.de

CECAD Lipidomics/Metabolomics Facility
CECAD Research Center
University of Cologne
Joseph-Stelzmann-Str. 26
50931 Köln
Profile

Figures

Figure 1
LC/MS/MS separation of sphingolipids from mouse liver: This approach includes the pre-separation of the lipid extract via a LC system. Lipids are detected at the QTRAP 6500 by Multiple Reaction Monitoring (MRM). Each peak represents a ceramide, glucosylceramide or sphingomyelin species acylated by a distinct fatty acid.

Figure 2
Shotgun Lipidomics analysis of glycerophospholipid subclasses (GPLs) from keratinocyte mitochondria (mitochondria provided by Rudolf Wiesner, Department of Physiology, Cologne). GPLs were simultaneously detected by applying specific precursor ion and neutral loss scans for each subclass (Multiple Precursor Ion Scanning, MPIS). Each peak in the mass spectrum represents a GPL species with a distinct fatty acid composition.

Prices

We charge our routine analyses. Graduated charges apply for the different user groups (CECAD members, members of the University of Cologne, external scientists). For inquiries please contact Dr. Susanne Brodesser.

 

Selected Publications
  • Yu H, Dilbaz S, Coßmann J, Hoang AC, Diedrich V, Herwig A, Harauma A, Hoshi Y, Moriguchi T, Landgraf K, Körner A, Lucas C, Brodesser S, Balogh L, Thuróczy J, Karemore G, Kuefner MS, Park EA, Rapp C, Travers JB, Röszer T. (2019) Breast milk alkylglycerols sustain beige adipocytes through adipose tissue macrophages. J Clin Invest 130:2485-99
  • Turpin-Nolan SM, Hammerschmidt P, Chen W, Jais A, Timper K, Awazawa M, Brodesser S, Brüning JC. (2019) CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes Obesity-Induced Insulin Resistance. Cell Rep 26(1):1-10
  • Späth MR, Bartram MP, Palacio-Escat N, Hoyer KJR, Debes C, Demir F, Schroeter CB, Mandel AM, Grundmann F, Ciarimboli G, Beyer A, Kizhakkedathu JN, Brodesser S, Göbel H, Becker JU, Benzing T, Schermer B, Höhne M, Burst V, Saez-Rodriguez J, Huesgen PF, Müller RU, Rinschen MM. (2019) The proteome microenvironment determines the protective effect of preconditioning in cisplatin-induced acute kidney injury. Kidney Int 95(2):333-49
  • Rashid T, Nemazanyy I, Paolini C, Tatsuta T, Crespin P, de Villeneuve D, Brodesser S, Benit P, Rustin P, Baraibar MA, Agbulut O, Olivier A, Protasi F, Langer T, Chrast R, de Lonlay P, de Foucauld H, Blaauw B, Pende M. (2019) Lipin1 deficiency causes sarcoplasmic reticulum stress and chaperone-responsive myopathy. EMBO J 38(1):e99576
  • Edifizi D, Nolte H, Babu V, Castells-Roca L, Mueller MM, Brodesser S, Krüger M, Schumacher B. (2017) Multilayered Reprogramming in Response to Persistent DNA Damage in C. elegans. Cell Rep 20(9):2026-43