For more than 10 years, the Facility has offered a comprehensive service for the analysis of lipids in biological samples using state-of-the-art chromatographic and mass spectrometric (MS) techniques. Since the beginning of 2019, we have expanded our expertise to the analysis of polar and ionic metabolites. Our service covers the entire workflow from sample preparation and MS measurement of the relevant metabolites to processing and analysis of the raw data obtained. Since 2016, the CECAD Lipidomics/Metabolomics Facility has been registered in the DFG portal "RIsources", a portal for research infrastructures.
Dr. Susanne Brodesser, Dipl. Chem.
LC/MS/MS separation of sphingolipids from mouse liver: This approach includes the pre-separation of the lipid extract via a LC system. Lipids are detected at the QTRAP 6500 by Multiple Reaction Monitoring (MRM). Each peak represents a ceramide, glucosylceramide or sphingomyelin species acylated by a distinct fatty acid.
Shotgun Lipidomics analysis of glycerophospholipid subclasses (GPLs) from keratinocyte mitochondria (mitochondria provided by Rudolf Wiesner, Department of Physiology, Cologne). GPLs were simultaneously detected by applying specific precursor ion and neutral loss scans for each subclass (Multiple Precursor Ion Scanning, MPIS). Each peak in the mass spectrum represents a GPL species with a distinct fatty acid composition.
We charge our routine analyses. Graduated charges apply for the different user groups (CECAD members, members of the University of Cologne, external scientists). For inquiries please contact Dr. Susanne Brodesser.