Technical Equipment

The CECAD Lipidomics/Metabolomics Facility houses three new-generation mass spectrometers (Orbitrap Exploris 240, Q Exactive HF, QTRAP 6500), including different UHPLC systems and two chip-based infusion systems for nano-electrospray ionization (TriVersa NanoMate). In addition, the Facility has recently received funding for a new triple quadrupole mass spectrometer, which is currently being procured.

Orbitrap Exploris 240

The Orbitrap Exploris 240 (Thermo Scientific) is a hybrid quadrupole/orbitrap mass spectrometer. It is coupled to a Vanquish Horizon Binary UHPLC and provides high-resolution mass spectra with high mass accuracy. The AcquireX™ intelligent data acquisition workflow of the Orbirap Exploris 240 facilitates the identification of unique and relevant metabolites in broad metabolomics analyses.

Q Exactive HF

The Q Exactive HF mass spectrometer (Thermo Scientific) is the predecessor model of the Orbitrap Exploris system. Our Q Exactive HF is coupled to two LC systems, the Vanquish Horizon Binary UHPLC and the Dionex Integrion RFIC (Thermo Scientific). The Integrion is a Reagent-Free Ion Chromatography (RFIC) system, which facilitates the separation and analysis of isomeric anionic compounds, e.g. the sugar phosphates of the glycolysis and organic acids of the TCA cycle.

QTRAP 6500

The QTRAP 6500 System (SCIEX) is a hybrid quadrupole/linear ion trap mass spectrometer. Due to its high sensitivity, it is particularly suitable for targeted quantification analyses. For LC-MS/MS analyses, we couple the QTRAP 6500 system to a 1260 Infinity Binary LC (Agilent) or to a Nexera X2 UHPLC (Shimadzu). In Shotgun Lipidomics analyses, the samples are directly infused using the TriVersa NanoMate (Advion).

Dr. rer. nat. Susanne Brodesser CECAD

Dr. rer. nat. Susanne Brodesser

Head of the Lipidomics/Metabolomics Facility 

+49 221 478 840 15


CECAD Lipidomics/Metabolomics Facility

CECAD Research Center
University of Cologne
Joseph-Stelzmann-Str. 26

50931 Köln