Q Exactive HF System:
The Q Exactive HF System (Thermo Scientific) is a hybrid-quadrupole/orbitrap mass spectrometer, which provides high-resolution mass spectra with high mass accuracy.
The Q Exactive HF is coupled to two LC systems, the Vanquish Horizon Binary UHPLC and the Dionex Integrion RFIC (Thermo Scientific). The Integrion is a Reagent-Free Ion Chromatography (RFIC) system, which enables the separation and analysis of ionic and polar compounds. The eluent does not have to be prepared manually beforehand, but is generated by the system's eluent generator.
We use the Q Exactive HF System for the targeted quantification and non-targeted profiling of anionic/polar metabolites and lipids.
QTRAP 6500 System:
The QTRAP 6500 System (SCIEX) is a mass spectrometer with a hybrid analyzer comprising a Triple Quadrupole (QQQ) analyzer and Linear Ion Trap (LIT). It is equipped with a Turbo V ion source which enables Electrospray Ionization (ESI) as well as Atmospheric Pressure Chemical Ionization (APCI).
For LC/MS/MS analyses, the QTRAP 6500 system is coupled to a 1260 Infinity Binary LC (Agilent) or to a Nexera X2 UHPLC (Shimadzu). In Shotgun Lipidomics analyses, the samples are infused via the TriVersa NanoMate (Advion), a chip-based infusion system for the generation of Nano-ESI.
The QTRAP 6500 System is used for the targeted quantification of lipids and polar metabolites.
TripleTOF 6600 System:
The TripleTOF 6600 System (SCIEX) is a high-resolution mass spectrometer with a Quadrupol-Time of Flight (Q-ToF) analyzer. As with the QTRAP 6500 System the ionization is implemented by ESI or APCI.
The 1260 Infinity Binary LC system as well as the Nexera X2 UHPLC (see QTRAP 6500) can be coupled to the TripleTOF 6600 System.
Devices for Thin Layer Chromatography (TLC):
For TLC analyses several devices of CAMAG are available at the Lipidomics/Metabolomics Facility: Using the Linomat 5, samples can be semi-automatically applied to TLC plates. The TLC Scanner 3 allows the quantification of lipid bands on the TLC by densitometry.
Dr. Susanne Brodesser, Dipl. Chem.
CECAD Lipidomics/Metabolomics Facility
CECAD Research Center
University of Cologne