Lipidomics/Metabolomics Facility

LC/MS separation of nucleotides and of intermediates of the glycolysis and the citric acid cycle: Metabolites are separated by Anion-Exchange Chromatography and detected using High-Resolution Mass Spectrometry (IC-HRMS)
LC/MS separation of nucleotides and of intermediates of the glycolysis and the citric acid cycle: Metabolites are separated by Anion-Exchange Chromatography and detected using High-Resolution Mass Spectrometry (IC-HRMS)

 

The facility offers a comprehensive service for the analysis of lipids and polar/ionic metabolites in biological samples using state-of-the-art chromatographic and mass spectrometric (MS) techniques.

Our service covers the entire workflow from sample preparation and MS measurement of the relevant metabolites to processing and analysis of the raw data obtained. Since 2016, the CECAD Lipidomics/Metabolomics Facility has been registered in the DFG portal "RIsources", a portal for research infrastructures.

Scheme of the Service Workflow
Service Workflow

Publications Highlights

  1. Tighanimine K, Nabuco Leva Ferreira Freitas JA, Nemazanyy I, Bankolé A, Benarroch-Popivker D, Brodesser S, Doré G, Robinson L, Benit P, Ladraa S, Saada YB, Friguet B, Bertolino P, Bernard D, Canaud G, Rustin P, Gilson E, Bischof O, Fumagalli S, Pende M. (2024) A homoeostatic switch causing glycerol-3-phosphate and phosphoethanolamine accumulation triggers senescence by rewiring lipid metabolism. Nat Metab 6(2):323-342. doi: 10.1038/s42255-023-00972-y.
     
  2. Moschandrea C, Kondylis V, Evangelakos I, Herholz M, Schneider F, Schmidt C, Yang M, Ehret S, Heine M, Jaeckstein MY, Szczepanowska K, Schwarzer R, Baumann L, Bock T, Nikitopoulou E, Brodesser S, Krüger M, Frezza C, Heeren J, Trifunovic A, Pasparakis M. (2024) Mitochondrial dysfunction abrogates dietary lipid processing in enterocytes. Nature 625(7994):385-392. doi: 10.1038/s41586-023-06857-0.
     
  3. Schütter M, Giavalisco P, Brodesser S, Graef M. (2020) Local fatty acid channeling into phospholipid synthesis drives phagophore expansion during autophagy. Cell 180(1):135-149.e14. doi: 10.1016/j.cell.2019.12.005.
     
  4. Sawa-Makarska J, Baumann V, Coudevylle N, von Bülow S, Nogellova V, Abert C, Schuschnig M, Graef M, Hummer G, Martens S. (2020) Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation. Science 369(6508):eaaz7714. doi: 10.1126/science.aaz7714.
     
  5. Hammerschmidt P, Ostkotte D, Nolte H, Gerl MJ, Jais A, Brunner HL, Sprenger HG, Awazawa M, Nicholls HT, Turpin-Nolan SM, Langer T, Krüger M, Brügger B, Brüning JC. (2019) CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity. Cell 177(6):1536- 1552.e23. doi: 10.1016/j.cell.2019.05.008.

Dr. rer. nat. Susanne Brodesser CECAD

Dr. rer. nat. Susanne Brodesser

Head of the Lipidomics/Metabolomics Facility 

+49 221 478 840 15

susanne.brodesser[at]uk-koeln.de

CECAD Lipidomics/Metabolomics Facility

CECAD Research Center
University of Cologne
Joseph-Stelzmann-Str. 26

50931 Köln

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Prices

We charge our routine analyses. Graduated charges apply for the different user groups (CECAD members, members of the University of Cologne, external scientists). For inquiries please contact Dr. Susanne Brodesser.

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