For more than 10 years, the Facility has offered a comprehensive service for the analysis of lipids in biological samples using state-of-the-art chromatographic and mass spectrometric (MS) techniques. Since the beginning of 2019, we have expanded our expertise to the analysis of polar and ionic metabolites. Our service covers the entire workflow from sample preparation and MS measurement of the relevant metabolites to processing and analysis of the raw data obtained. Since 2016, the CECAD Lipidomics/Metabolomics Facility has been registered in the DFG portal "RIsources", a portal for research infrastructures.
Figure 1: LC/MS separation of nucleotides and of intermediates of the glycolysis and the citric acid cycle: Metabolites are separated by Anion-Exchange Chromatography and detected using High-Resolution Mass Spectrometry (IC-HRMS)
Figure 2: Shotgun Lipidomics analysis of glycerophospholipids (GPLs) from B lymphocytes:
GPL subclasses are simultaneously detected by applying specific precursor ion and neutral loss scans for each subclass. Each peak in the mass spectrum represents GPL species with a distinct fatty acid composition.
We charge our routine analyses. Graduated charges apply for the different user groups (CECAD members, members of the University of Cologne, external scientists). For inquiries please contact Dr. Susanne Brodesser.